Studies on Ribonucleic Acid Turnover in Rat Organs
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چکیده
منابع مشابه
Studies on the Solubilized Ribonucleic Acid Polymerase from Rat
1. By using ultrasonic treatment in media of high ionic strength, the RNA polymerase activities associated with prostatic nuclei and nucleoli can be completely solubilized. Such enzyme preparations are entirely dependent on the provision of added DNA for full activity. 2. The solubilized enzymes from the nucleolar and extranucleolar regions can be separated by ion-exchange chromatography. 3. Ba...
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A method for the estimation of DNA and RNA in rat brain is described. The principal modifications of preexisting methods involve initial lipid extraction with chloroformmethanol and continuous stirring during alkaline hydrolysis of RNA and acid extraction of DNA. Comparison of ultraviolet-absorptive methods, phosphate determination, and orcinol reaction for RNA, shows a greater degree of corres...
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A procedure for the isolation and purification of bacteriophage T34nduced RNA polymerase from T34nfected Escherichia coli cells is presented. The procedure described leads to the isolation of polymerase preparation that displays a single protein band by polyacrylamide gel electrophoresis and is free of detectable ribonuclease and deoxyribonuclease activities. Other enzyme activities absent from...
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Rat chromosomes vary considerably in volume from one normal cell type to another. The relationship of their size to the concentration of the B vitamins and ribonucleic acid, and to nuclear volurne, should aid in explaining the significance of the morphological differences between chromosomes of cancer cells and those of normal tissues (2, 6, 7) that are already evident by the third day of epide...
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Strong evidence has accumulated in recent years in favor of the following scheme of protein biosynthesis. DNA directs the formation of complementary strands of messenger RNA which possess the necessary genetic information to determine the sequential arrangement of amino acids in polypeptide chains (1, 2). The messenger RNA attaches itself to ribosomes to form a multiple ribosomal structure, the...
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ژورنال
عنوان ژورنال: Endocrinologia Japonica
سال: 1972
ISSN: 0013-7219,2185-6370
DOI: 10.1507/endocrj1954.19.225